Human ACTN3 (Actinin Alpha 3) ELISA Kit | G-EC-02346

Human ACTN3 (Actinin Alpha 3) ELISA Kit | G-EC-02346 | Gentaur Elisa Kits

Target Species: Human

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivity: 0.19ng/mL

Detection Range: 0.31~20ng/mL

UniProt ID: Q08043

Target Name: ACTN3

Target Synonym: alpha-actinin skeletal muscle isoform 3, F-actin cross-linking protein, MGC117002, MGC117005

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ACTN3. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ACTN3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ACTN3, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human ACTN3. You can calculate the concentration of Human ACTN3 in the samples by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Human ACTN3 in samples. No significant cross-reactivity or interference between Human ACTN3 and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Human ACTN3 concentrations in Serum, plasma and other biological fluids.

Research Areas: Cancer, Signal transduction

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Human ACTN3 (Actinin Alpha 3) ELISA Kit DataSheet