Human HIF-1α (Hypoxia Inducible Factor 1 Alpha) CLIA Kit | G-EC-00794

Human HIF-1α (Hypoxia Inducible Factor 1 Alpha) CLIA Kit | G-EC-00794 | Gentaur Clia Kits

Target Species: Human

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 18.75pg/mL

Detection Range: 31.25~2000pg/mL

UniProt ID:

Target Name: HIF-1α

Target Synonym: HIF1A, HIF-1A, MOP1, PASD8, bHLHe78, basic helix-loop-helix transcription factor

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HIF-1α. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HIF-1α. You can calculate the concentration of Human HIF-1α in the samples by comparing the RLU value of the samples to the standard curve.

Specificity: This kit recognizes Human HIF-1α in samples. No significant cross-reactivity or interference between Human HIF-1α and analogues was observed.

Application: This CLIA kit applies to the in vitro quantitative determination of Human HIF-1α concentrations in Serum, plasma and other biological fluids.

Research Areas: Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Human HIF-1α (Hypoxia Inducible Factor 1 Alpha) CLIA Kit DataSheet