METTL16 Polyclonal Antibody | G-AB-09241

METTL16 Polyclonal Antibody | G-AB-09241 | Gentaur Antibodies

Overview: RNA N6-methyltransferase that methylates adenosine residues at the N (6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753, PubMed:30197299, PubMed:30197297) . Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs. In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure. Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing and/or stability of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, impairing MAT2A expression. In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A. In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA) : specifically N6-methylates adenine in position 43 of U6 snRNAs. Also able to bind various lncRNAs, such as 7SK snRNA (7SK RNA) or 7SL RNA. Specifically binds the 3'-end of the MALAT1 long non-coding RNA.

Category Type: Polyclonal Antibody

Research Areas:

Synonyms: METTL16, METT10D

Reactivity: Human, Mouse, Rat

Host: Rabbit

Isotype: IgG

Gene ID: 79066

Accession #:

Clonality:

Immunogen: Recombinant fusion protein of human METTL16 (NP_076991.3) .

Clone #:

Conjugation: Unconjugated

Swissprot: Q86W50

Santa Cruz:

Calculated MW:

Observed MW:

Concentration: 1 mg/mL

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Purification method: Affinity purification

Application: IF

Dilution: IF 1:50-1:200

Storage: Store at -20°C. Avoid freeze / thaw cycles.

METTL16 Polyclonal Antibody DataSheet