Mouse APLN (Apelin) ELISA Kit | G-EC-00153

Mouse APLN (Apelin) ELISA Kit | G-EC-00153 | Gentaur Elisa Kits

Target Species: Mouse

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 75pg/mL

Detection Range: 125~8000pg/mL

UniProt ID: Q9R0R4

Target Name: APLN

Target Synonym: APEL, XNPEP2

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Mouse APLN. During the reaction, Mouse APLN in samples or Standard competes with a fixed amount of Mouse APLN on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Mouse APLN. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Mouse APLN in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Mouse APLN in samples. No significant cross-reactivity or interference between Mouse APLN and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Mouse APLN concentrations in Serum, plasma and other biological fluids.

Research Areas: Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Mouse APLN (Apelin) ELISA Kit DataSheet