P5 (Pregnenolone) ELISA Kit | G-EC-00044

P5 (Pregnenolone) ELISA Kit | G-EC-00044 | Gentaur Elisa Kits

Target Species: Universal

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 0.47ng/mL

Detection Range: 0.78~50ng/mL

UniProt ID:

Target Name: Pregnenolone

Target Synonym:

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Pregnenolone. During the reaction, Pregnenolone in samples or Standard competes with a fixed amount of Pregnenolone on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Pregnenolone. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Pregnenolone in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Pregnenolone in samples. No significant cross-reactivity or interference between Pregnenolone and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Pregnenolone concentrations in Serum, plasma and other biological fluids.

Research Areas: Neuroscience, Developmental biology

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

P5 (Pregnenolone) ELISA Kit DataSheet

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