Rat ALPI (Intestinal alkaline phosphatase 1) ELISA Kit | G-EC-05172

Rat ALPI (Intestinal alkaline phosphatase 1) ELISA Kit | G-EC-05172 | Gentaur Elisa Kits

Target Species: Rat

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivity: 0.47ng/mL

Detection Range: 0.78~50ng/mL

UniProt ID: P15693

Target Name: ALPI

Target Synonym: Calf Intestinal Alkaline Phosphatase, CIP, IAP,

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ALPI. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ALPI and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ALPI, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Rat ALPI. You can calculate the concentration of Rat ALPI in the samples by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Rat ALPI in samples. No significant cross-reactivity or interference between Rat ALPI and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Rat ALPI concentrations in Serum, plasma and other biological fluids.

Research Areas: Cancer, Cell Biology, Metabolism, Immunology, Signal transduction

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Rat ALPI (Intestinal alkaline phosphatase 1) ELISA Kit DataSheet