Anti GAPDH Antibody-HRP
Anti GAPDH Antibody-HRP
HRP-conjugated monoclonal antibody for detection of GAPDH, a commonly used reference protein in Western blot and molecular biology workflows.
Product Overview
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a key enzyme involved in glycolysis. It catalyzes the oxidation and phosphorylation of glyceraldehyde-3-phosphate, producing 1,3-bisphosphoglycerate.
GAPDH is widely distributed across tissues and is commonly used as a reference protein in molecular biology experiments because of its generally abundant expression. It is frequently used as an internal reference in applications such as Western blot (WB) and qPCR.
Anti GAPDH Antibody-HRP is produced by conjugating Horseradish Peroxidase (HRP) to a GAPDH antibody. The HRP conjugate enables direct chemiluminescent detection of GAPDH during Western blot analysis.
Key Features
GAPDH Detection
Designed for detection of GAPDH reference protein in Western blot workflows.
HRP Conjugated
HRP conjugation supports direct chemiluminescent detection using an appropriate substrate system.
Broad Cross-Reactivity
Listed cross-reactivity includes human, rat, mouse, plant, yeast, and other species.
Product Specifications
| Antibody Source | Recombinant antibody |
| Cross Reactivity | Human, rat, mouse, plant, yeast, etc. |
| Clone Type | Monoclonal antibody |
| Conjugate | Horseradish Peroxidase (HRP) |
| Purification Method | rProtein A affinity purification |
| Storage Buffer | 1× PBS (pH 7.4), 0.05% ProClin 300, 50% glycerol |
Storage & Handling
Shipping: Shipped with dry ice.
Storage: Stable for 2 years at −20°C.
Handling: Avoid repeated freeze/thaw cycles.
HRP Chemiluminescent Detection
During Western blot detection, luminol and hydrogen peroxide are used as chemiluminescent substrates. In the presence of HRP, luminol reacts with hydrogen peroxide to generate an unstable peroxide intermediate.
The intermediate rapidly decomposes through an electronically excited state. When the excited species returns to its ground state, light is emitted, allowing visualization of the GAPDH reference protein.
Western Blot Workflow
Separate proteins by electrophoresis.
Transfer separated proteins onto a membrane.
Apply the HRP-conjugated GAPDH antibody.
Add chemiluminescent substrate and visualize the signal.
Ordering Information
| Product | Cat. No. | Size |
|---|---|---|
| Anti GAPDH Antibody-HRP | BP5018-01 | 20 µL |
| Anti GAPDH Antibody-HRP | BP5018-02 | 100 µL |
| Anti GAPDH Antibody-HRP | BP5018-03 | 1 mL |
Reliable GAPDH Detection for Western Blot
Anti GAPDH Antibody-HRP provides a convenient HRP-conjugated format for detecting GAPDH reference protein in Western blot experiments.
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