Diagnostic Microspheres

1. Chemiluminescence Immunoassay

Self-developed Anti-CA153-093 mAb was conjugated toCarboxyl-Activated MagPoly Beads using a two-step method. Key performance evaluations, including reaction kinetics, precision, and clinical correlation, were conducted in an acridinium ester direct chemiluminescence system (Instrument: Yingkai 12910).

Figure 1 Research on the performance of Carboxyl-Activated MagPoly Beads (Take the CA153 project as an example)

2. Latex Immunoturbidimetry

Various antibodies were modified onto different latex microsphere products  via activation, conjugation, and blocking steps. Reactivity was assessed on a fully automated biochemical analyzer (Instrument: Hitachi 7180).

cTnI

Figure 2.1 Detection range: 0.5-25.0ng/ml

PCT

Figure 2.1 Detection range: 0.2-25.0ng/ml

D-dimer

Figure 2.3 Detection range: 0.2-20.0μg/ml

3. Immunochromatographic Assay

Depending on your application direction and specific needs, carriers such as colloidal gold, time-resolved microspheres, or fluorescent microspheres can be selected for protein/antibody conjugation and modification to qualitatively or semi-quantitatively evaluate raw material performance.

1. High binding capacity for biotin-modified ligands

As shown in the Figure 1, the Ct values of mRNA before and after purification are essentially consistent, indicating that the purification efficiency of mRNA is close to 100% and the non-specific binding is very low. (Actual results may vary due to differences in experimental conditions, detection instruments, etc. The data in the figure are for reference only.)

Figure 1. Results of using Oligo (dT)25 Magnetic Beads for purifying mRNA from total RNA( mouse liver).

2. Good batch-to-batch stability

Three batches of Oligo(dT)25 MagPoly Beads were each incubated and hybridized with the same total RNA sample to test mRNA and rRNA capture. CT value comparison with the sample indicates low batch-to-batch variation in mRNA purification, demonstrating high application stability.

1. High binding capacity

Under identical conditions, antibodies were purified using resin and magarose beads, respectively. As indicated by the yield(Figure 1.1) and purity(Figure 1.2) of the purified products, the capture and binding capacity of the magnetic beads is highly comparable to that of the resin.

Figure 1.1 Results of binding capacity

2. High-throughput Application Case

Different sample types (cell culture supernatant and rabbit serum) were incubated with  rProtein A/G MagPoly Beads(Cat\:SM01510) for automated purification on the Purifier 24(Cat\:P241001). For the cell culture supernatant, parallel experiments were conducted with varying concentrations and volumes. The purity and recovery yield of the purified products were both ≥90%（Table 1, Figure 2).

Table 1 Results of Recovery yield and Purity

Cell culture medium(4ml)

Figure 2 Results of Purity( SDS-PAGE)