Human HSP gp96 (Heat Shock Protein glycoprotein 96) ELISA Kit | G-EC-03318

Human HSP gp96 (Heat Shock Protein glycoprotein 96) ELISA Kit | G-EC-03318 | Gentaur Elisa Kits

Target Species: Human

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivity: 0.19ng/mL

Detection Range: 0.31~20ng/mL

UniProt ID: P14625

Target Name: HSP gp96

Target Synonym: Heat shock protein 90kDa beta member 1, HSP90B1, endoplasmin, gp96, grp94, ERp99

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HSP gp96. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP gp96 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP gp96, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human HSP gp96. You can calculate the concentration of Human HSP gp96 in the samples by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Human HSP gp96 in samples. No significant cross-reactivity or interference between Human HSP gp96 and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Human HSP gp96 concentrations in Serum, plasma and other biological fluids.

Research Areas: Cancer, Metabolism, Signal transduction, Tags and Cell Markers

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Human HSP gp96 (Heat Shock Protein glycoprotein 96) ELISA Kit DataSheet