Mouse Anti- (PVL LukS) mAb (1D9) | 494-0221-001
Catalog #:
0221-001
Immunogen:
Recombinant LukS-PV (His tag)
Description:
Mouse monoclonal antibody reactive to a conformational epitope of Staphylococcus aureus Panton Valentine Leukocidin LukS subunit.
Supplied:
100 µg is supplied in PBS at a concentration of 3.98
mg/mL.
Purification:
Antibody is purified using immobilized protein A.
Clonality:
Monoclonal of the IgG2b isotype.
Relevance:
The antibody can be used for detection of LukS-PV in an ELISA sandwich assay. It is not recommended for denatured Western blot detection due to the recognition of a partially conformational epitope of the LukS.
ELISA:
Assay-dependent dilution. Internal QC demonstrates good capture of purified recombinant LukS-PV using this monoclonal antibody coated to a plate at 100 ng/well.
WB:
It is not recommended to use this antibody for Western Blotting application due to weak detection of LukS-PV.
Cross Reactivity:
As with most antibodies, this antibody may interact with Protein A in S. aureus culture supernatant via the Fc region. Other cross reactive epitopes are not known at this time.
Storage:
2-3 weeks +4oC, -20â—¦C long term
