PGE1 (Prostaglandin E1) ELISA Kit | G-EC-00024

PGE1 (Prostaglandin E1) ELISA Kit | G-EC-00024 | Gentaur Elisa Kits

Target Species: Universal

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 46.88pg/mL

Detection Range: 78.13~5000pg/mL

UniProt ID:

Target Name: PGE1

Target Synonym: Prostaglandin E1, PGE1, alprostadil

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGE1. During the reaction, PGE1 in samples or Standard competes with a fixed amount of PGE1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PGE1. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of PGE1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes PGE1 in samples. No significant cross-reactivity or interference between PGE1 and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of PGE1 concentrations in Serum, plasma and other biological fluids.

Research Areas: Cell Biology

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

PGE1 (Prostaglandin E1) ELISA Kit DataSheet