5-HETE (5-Hydroxyeicosatetraenoic Acid) ELISA Kit | G-EC-00051

5-HETE (5-Hydroxyeicosatetraenoic Acid) ELISA Kit | G-EC-00051 | Gentaur Elisa Kits

Target Species: Universal

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 0.09ng/mL

Detection Range: 0.16~10ng/mL

UniProt ID:

Target Name: 5-HETE

Target Synonym:

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 5-HETE. During the reaction, 5-HETE in samples or Standard competes with a fixed amount of 5-HETE on the solid phase supporter for sites on the Biotinylated Detection Ab specific to 5-HETE. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of 5-HETE in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes 5-HETE in samples. No significant cross-reactivity or interference between 5-HETE and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of 5-HETE concentrations in Serum, plasma and other biological fluids.

Research Areas: Signal transduction, Metabolism, Cardiovascular, Cardiovascular

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

5-HETE (5-Hydroxyeicosatetraenoic Acid) ELISA Kit DataSheet