Sepharose CL-2B
21st Aug 2026
Sepharose CL-2B
A well-proven cross-linked agarose gel filtration base matrix frequently used for coupling affinity ligands to the matrix.
Product Overview
Sepharose CL-2B is a well-proven cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.
The matrix is not pre-activated and the user performs all steps in coupling.
Description
Sepharose is a bead-formed agarose-based gel filtration matrix. Sepharose is available with 3 different agarose contents; 2%, 4%, and 6%, designated Sepharose 2B, Sepharose 4B and Sepharose 6B respectively.
Both Sepharose and Sepharose CL have broad fractionation ranges which makes them suitable for characterizing or cleaning-up samples containing components of diverse molecular weight.
Key Characteristics
| Matrix | 2% agarose |
| Particle Size | 60 μm–200 μm |
| Recommended Flow Rate | <15 cm/h |
| Recommended Column Height | 10 cm |
| pH Working Range | 3–13 |
| CIP Stability | pH 2–14 |
Product Specifications
| Specification | Details |
| Product | Sepharose CL-2B |
| Catalog No. | Se-500C |
| Matrix | 2% agarose |
| Particle Size | 60 μm–200 μm |
| Recommended Flow Rate | <15 cm/h |
| Recommended Column Height | 10 cm |
| Maximum Operating Pressure | 60–120 cm/h |
| Pressure Drop | 15 cm H₂O / bed height |
| Bed Height | 10 cm |
| Column Internal Diameter | 5 cm |
| pH Working Range | 3–13 |
| CIP Stability | pH 2–14 |
| Autoclavable | Yes |
| Sterilization | 20 min at 120°C in phosphate buffer, pH 7 |
| Storage | 4 to 30°C, 20% Ethanol |
| Pack Size | 1 L |
Equilibration
Before applying the sample, equilibrate the column with at least two column volumes of the eluent to be used in the separation, or until the baseline is stable.
Longer equilibration might be needed with detergent solutions. Equilibration is not needed between runs with the same eluent.
Regeneration
Regeneration is normally performed by washing with 2 to 3 column volumes of buffer, followed by re-equilibration in the new buffer if changing conditions.
Sanitization
Wash the column with 0.5 M NaOH.