CALR Polyclonal Antibody | G-AB-07430

CALR Polyclonal Antibody | G-AB-07430 | Gentaur Antibodies

Overview: Calreticulin is a multifunctional protein that acts as a major Ca (2+) -binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium. The amino terminus of calreticulin interacts with the DNA-binding domain of the glucocorticoid receptor and prevents the receptor from binding to its specific glucocorticoid response element. Calreticulin can inhibit the binding of androgen receptor to its hormone-responsive DNA element and can inhibit androgen receptor and retinoic acid receptor transcriptional activities in vivo, as well as retinoic acid-induced neuronal differentiation. Thus, calreticulin can act as an important modulator of the regulation of gene transcription by nuclear hormone receptors. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin but calreticulin is not a Ro/SS-A antigen. Earlier papers referred to calreticulin as an Ro/SS-A antigen but this was later disproven. Increased autoantibody titer against human calreticulin is found in infants with complete congenital heart block of both the IgG and IgM classes.

Category Type: Polyclonal Antibody

Research Areas: Cancer, Signal Transduction, Tags & Cell Markers

Synonyms: CALR, CRT, HEL-S-99n, RO, SSA, cC1qR

Reactivity: Human, Mouse, Rat

Host: Rabbit

Isotype: IgG

Gene ID: 811

Accession #:

Clonality:

Immunogen: Recombinant fusion protein of human CALR (NP_004334.1) .

Clone #:

Conjugation: Unconjugated

Swissprot: P27797

Santa Cruz:

Calculated MW: 48 kDa

Observed MW: 55 kDa

Concentration: 1 mg/mL

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3

Purification method: Affinity purification

Application: WB, IHC, IF

Dilution: WB 1:500-1:2000 IHC 1:50-1:200 IF 1:20-1:100

Storage: Store at -20°C. Avoid freeze / thaw cycles.

CALR Polyclonal Antibody DataSheet