ECL Basic / Enhanced / Super
ECL Basic / Enhanced / Super
A luminol-based chemiluminescence reagent system for HRP-mediated protein detection, designed to provide strong signal intensity, reduced background interference and convenient room-temperature storage.
Product Overview
ECL Basic, ECL Enhanced and ECL Super are chemiluminescence reagent systems based on a luminol substrate. In the presence of horseradish peroxidase (HRP), the working detection solution generates light emission that can be captured using appropriate imaging or exposure systems.
The optimized substrate formulation incorporates a high-efficiency enhancer designed to increase luminescence intensity while helping reduce background interference. According to the supplied product information, the resulting signal can be approximately 30–100 times higher than traditional ECL developing solutions, depending on the formulation and detection conditions.
A new oxidizing system replaces unstable hydrogen peroxide in the formulation, contributing to improved reagent stability. The kit can be stored at room temperature for up to one year under the recommended storage conditions.
Key Features
Chemiluminescent substrate system activated by HRP for protein detection.
Optimized enhancer technology provides high luminescence intensity and supports sensitive detection.
Formulation designed to improve signal-to-background performance during chemiluminescent detection.
Can be stored at room temperature for up to one year according to the supplied product information.
Detection Principle
HRP catalyzes the chemiluminescent reaction of the luminol-based working solution.
The reaction produces chemiluminescent light in the approximately 400–450 nm range.
The emitted signal can be captured using X-ray film or a suitable fluorescence CCD imaging system.
Applications
Chemiluminescent detection of HRP-labeled proteins following Western blot workflows.
Suitable for immunoassay systems using HRP-mediated chemiluminescent detection.
Compatible with X-ray film exposure and direct imaging using suitable CCD systems.
Storage Conditions
Store the ECL reagent kit at room temperature. According to the supplied product information, the kit can be stored for up to one year under the recommended conditions.
Ordering Information
| Product | Cat. No. | Size |
|---|---|---|
| ECL Basic | BK0040-01 | 50 mL + 50 mL |
| ECL Basic | BK0040-02 | 250 mL + 250 mL |
| ECL Enhanced | BK0041-01 | 50 mL + 50 mL |
| ECL Enhanced | BK0041-02 | 250 mL + 250 mL |
| ECL Super | BK0042-01 | 50 mL + 50 mL |
Need an ECL Chemiluminescence Reagent?
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