Sensitivity: 0.188 ng/mL (The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero).
Detection Range: 0.313–20 ng/mL
Specificity: This kit recognizes Biotin. No significant cross-reactivity or interference was observed.
Repeatability: Coefficient of variation is <10%.

Test principle

This kit uses Competitive-method. The microplate provided in this kit has been pre-coated with Biotin. During the reaction, Biotin in the sample or standard competes with a fixed amount of Biotin on the solid phase supporter for sites on the Avidin-HRP specific to Biotin. Excess Avidin-HRP and unbound sample or standard are washed from the plate. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by adding Stop Solution and the color change can be measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Biotin in samples can be calculated by comparing the OD of the samples with the standard curve.

Calculation of results

Average the duplicate readings for each standard and samples. Plot a four-parameter logistic curve on log-log graph paper, with standard concentration on the x-axis and OD values on the y-axis.

If samples have been diluted, the concentration calculated from the standard curve must be multiplied by the dilution factor. If the OD of the sample surpasses the lower limit of the standard curve, you should re-test it after appropriate dilution. The actual concentration is the calculated concentration multiplied dilution factor.

SUMMARY

1.  Add 50 μL standard or sample to each well. 

2.  Immediately add 50 μL Avidin-HRP to each well. 

3.  Incubate for 30 min at 37℃

4. Aspirate and wash 5 times

5. Add 90 μL Substrate Reagent. Incubate 15 min at 37℃

6. Add 50 μL Stop Solution. Read at 450 nm immediately.

 7. Calculation of results

 Kit components & Storage

The unopened kit can be stored at 4℃ for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions since the kit is received.

Note: All reagent bottle caps must be tightened to prevent evaporation and microbial pollution.

The volume of reagents in partial shipments is a little more than the volume marked on the label, please use in measuring instead of directly pouring.

Other supplies required

Microplate reader with 450nm wavelength filter

High-precision transferpettor, EP tubes and disposable pipette tips

37°C Incubator, Deionized or distilled water

Absorbent paper

otal Aflatoxin are mycotoxins produced by Aspergillus flavus and Aspergillus parasiticus due to some reasons, and they are widely distributed in nature. There are about 20 kinds of its derivatives, of which aflatoxin B1 has the strongest toxicity and the strongest carcinogenicity. Aflatoxin have been found in pet and human food and raw materials for agricultural animals.

As aflatoxin can reduce the productivity of livestock and poultry and cause liver necrosis, the U.S. FDA has set the maximum residue limit (MRL) of cereals and cereal products to 20 ppb, and the European Union (EU) has set the maximum residue limit (MRL) of edible oil to 4 ppb.

Compared with traditional methods,the Elabscience Total Aflatoxin kit is simple, fast, and sensitive, and is suitable for the detection of large quantities of samples.

Elabsciencefood safety kits are used to detect hormone residues, mycotoxins, toxic and harmful substances and antibiotic residues in feed, food and other sample. It can be widely used in food and drug Administration, health departments, various colleges, scientific research units, agricultural departments, animal husbandry and veterinarians, breeding farms, slaughterhouses, food and meat products deep processing enterprises, inspection and quarantine departments, etc.

It can meet the high requirements of consumers for food quality and the scientific researchers to obtain efficient experimental data. The kit is manufactured in accordance with the international quality standard ISO9001.

This ELISA kit uses the Sandwich-Ag ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with recombinant SARS-CoV-2 Spike Protein. After adding samples (or controls) to wells, the total antibodies against SARS-CoV-2 Spike Protein will combine with the pre-coated SARS-CoV-2 Spike Protein. After washing completely, add Horseradish Peroxidase (HRP) conjugated SARS-CoV-2 Spike Protein to develop the antigen-antibody-HRP conjugated antigen complex.

Free components are washed away. The substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Spike Protein, antibodies against SARS-CoV-2 Spike Protein and HRP conjugated SARS-CoV-2 Spike Protein will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike Protein total antibody exists in the tested samples or not.