Fast Transfer Buffer (20×)

Fast Transfer Buffer 20x
WESTERN BLOT & PROTEIN TRANSFER

Fast Transfer Buffer (20×)

A concentrated transfer buffer formulated for rapid and efficient protein transfer during Western blot workflows.

Concentration
20×
Transfer Time
40–60 min
Pack Size
500 mL
Request a Quote

Product Overview

Fast Transfer Buffer (20×) is a concentrated transfer solution designed to accelerate the protein transfer step in Western blot experiments. Its unique transfer formulation enables protein transfer to be completed in approximately 40–60 minutes.

Under comparable conditions, the use of this transfer buffer can reduce transfer time by more than half compared with traditional transfer solutions. It is formulated to provide effective transfer performance across proteins with different molecular weights.

Key Benefits

01

Rapid Protein Transfer

Enables the transfer step to be completed within approximately 40–60 minutes.

02

Time-Saving Workflow

Can save more than half of the transfer time compared with traditional transfer solutions under the same conditions.

03

Broad Protein Transfer

Designed to provide effective transfer performance for proteins with different molecular weights.

Application in Western Blotting

Fast Transfer Buffer (20×) is intended for the protein transfer stage following electrophoresis and prior to antibody-based detection. Its rapid transfer formulation helps shorten the overall Western blot workflow while maintaining transfer performance across different protein molecular weights.

Recommended Use

Before using Fast Transfer Buffer (20×), soak the gel in deionized water for 1–2 minutes.

For optimized workflow compatibility, this transfer buffer can be used together with ACE FuturePAGE™ protein pre-prepared gel products.

Western Blot Transfer Workflow

STEP 01
Protein Electrophoresis

Separate proteins using PAGE.

STEP 02
Gel Preparation

Soak the gel in deionized water for 1–2 minutes.

STEP 03
Protein Transfer

Use Fast Transfer Buffer for rapid transfer.

STEP 04
Western Blot Detection

Continue with blocking and antibody detection.

Ordering Information

Product Cat. No. Size
Fast Transfer Buffer (20×) BR0003-01 500 mL

Accelerate Your Western Blot Transfer Workflow

Fast Transfer Buffer (20×) provides a convenient solution for rapid protein transfer, helping reduce processing time in Western blot experiments.

Request a Quote