Human AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) CLIA Kit | G-EC-00272

Human AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) CLIA Kit | G-EC-00272 | Gentaur Clia Kits

Target Species: Human

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 7.5pg/mL

Detection Range: 12.5~800pg/mL

UniProt ID:

Target Name: AFGF/FGF1

Target Synonym: FGF-1, ECGF, ECGF-beta, A-FGF, FGFA, ECGFA, ECGFB, FGF-Alpha, HBGF1, HBGF-1, ECGFB, GLIO703, Heparin-binding growth factor 1

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AFGF/FGF1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AFGF/FGF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AFGF/FGF1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human AFGF/FGF1. You can calculate the concentration of Human AFGF/FGF1 in the samples by comparing the RLU value of the samples to the standard curve.

Specificity: This kit recognizes Human AFGF/FGF1 in samples. No significant cross-reactivity or interference between Human AFGF/FGF1 and analogues was observed.

Application: This CLIA kit applies to the in vitro quantitative determination of Human AFGF/FGF1 concentrations in Serum, plasma and other biological fluids.

Research Areas: Cancer, Cardiovascular, Neuroscience, Signal transduction

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Human AFGF/FGF1 (Acidic Fibroblast Growth Factor 1) CLIA Kit DataSheet