Human Ang-Ⅱ (Angiotensin Ⅱ) ELISA Kit | G-EC-00079
- 3 to 5 Working Days
Human Ang-Ⅱ (Angiotensin Ⅱ) ELISA Kit | G-EC-00079 | Gentaur Elisa Kits
Target Species: Human
Assay Time: 2.5h
Detection Type: Colormetric
Detection Range: 31.25~2000pg/mL
UniProt ID: P01019 (34~41aa)
Target Name: Ang-Ⅱ
Target Synonym: Angiotensin-2
Tested Sample Types: Serum, plasma and other biological fluids
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Ang-Ⅱ. During the reaction, Human Ang-Ⅱ in samples or Standard competes with a fixed amount of Human Ang-Ⅱ on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Ang-Ⅱ. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Human Ang-Ⅱ in the samples is then determined by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Human Ang-Ⅱ in samples. No significant cross-reactivity or interference between Human Ang-Ⅱ and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Human Ang-Ⅱ concentrations in Serum, plasma and other biological fluids.
Research Areas: Cancer, Cardiovascular, Metabolism, Signal transduction
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.