Human BNP (Brain Natriuretic Peptide) ELISA Kit | G-EC-00088

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Human BNP (Brain Natriuretic Peptide) ELISA Kit | G-EC-00088 | Gentaur Elisa Kits

Target Species: Human

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 18.75pg/mL

Detection Range: 31.25~2000pg/mL

UniProt ID: P16860

Target Name: BNP

Target Synonym: NPPB, GC-B, B-Type Natriuretic Peptide, Natriuretic Peptide Precursor B

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human BNP. During the reaction, Human BNP in samples or Standard competes with a fixed amount of Human BNP on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human BNP. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Human BNP in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Human BNP in samples. No significant cross-reactivity or interference between Human BNP and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Human BNP concentrations in Serum, plasma and other biological fluids.

Research Areas: Cancer, Cardiovascular, Neuroscience

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Human BNP (Brain Natriuretic Peptide) ELISA Kit DataSheet

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