Human CRP (C-Reactive Protein) ELISA Kit | G-EC-02225
- 3 to 5 Working Days
Human CRP (C-Reactive Protein) ELISA Kit | G-EC-02225 | Gentaur Elisa Kits
Target Species: Human
Assay Time: 3.5h
Detection Type: Colormetric
Detection Range: 0.39~25ng/mL
UniProt ID: P02741
Target Name: CRP
Target Synonym: PTX1, Pentraxin 1
Tested Sample Types: Serum, plasma and other biological fluids
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CRP. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human CRP. You can calculate the concentration of Human CRP in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Human CRP in samples. No significant cross-reactivity or interference between Human CRP and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Human CRP concentrations in Serum, plasma and other biological fluids.
Research Areas: Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Immunology
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.