Human IκBα (Inhibitory Subunit of NF Kappa B Alpha) CLIA Kit | G-EC-01189
- 3 to 5 Working Days
Human IκBα (Inhibitory Subunit of NF Kappa B Alpha) CLIA Kit | G-EC-01189 | Gentaur Clia Kits
Target Species: Human
Assay Time: 3.5h
Detection Type: Chemiluminescence
Detection Range: 31.25~2000pg/mL
Target Name: IκBα
Tested Sample Types: Serum, plasma and other biological fluids
Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IκBα. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IκBα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IκBα, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human IκBα. You can calculate the concentration of Human IκBα in the samples by comparing the RLU value of the samples to the standard curve.
Specificity: This kit recognizes Human IκBα in samples. No significant cross-reactivity or interference between Human IκBα and analogues was observed.
Application: This CLIA kit applies to the in vitro quantitative determination of Human IκBα concentrations in Serum, plasma and other biological fluids.
Research Areas: Cancer, Epigenetics and Nuclear Signaling, Immunology, Signal transduction
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.
Human IκBα (Inhibitory Subunit of NF Kappa B Alpha) CLIA Kit DataSheet