Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit | G-EC-00599

Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit | G-EC-00599 | Gentaur Clia Kits

Target Species: Human

Type: Sandwich

Assay Time: 3.5h

Detection Type: Chemiluminescence

Sensitivity: 9.38pg/mL

Detection Range: 15.63~1000pg/mL

UniProt ID:

Target Name: NOS3/eNOS

Target Synonym: ECNOS, NOS III, E-NOS

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NOS3/eNOS. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NOS3/eNOS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NOS3/eNOS, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NOS3/eNOS. You can calculate the concentration of Human NOS3/eNOS in the samples by comparing the RLU value of the samples to the standard curve.

Specificity: This kit recognizes Human NOS3/eNOS in samples. No significant cross-reactivity or interference between Human NOS3/eNOS and analogues was observed.

Application: This CLIA kit applies to the in vitro quantitative determination of Human NOS3/eNOS concentrations in Serum, plasma and other biological fluids.

Research Areas: Cancer, Cell Biology, Cardiovascular, Metabolism, Neuroscience

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Human NOS3/eNOS (Nitric Oxide Synthase 3, Endothelial) CLIA Kit DataSheet