Human SOD2 (Superoxide Dismutase 2, Mitochondrial) ELISA Kit | G-EC-00111

Human SOD2 (Superoxide Dismutase 2, Mitochondrial) ELISA Kit | G-EC-00111 | Gentaur Elisa Kits

Target Species: Human

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 0.19ng/mL

Detection Range: 0.31~20ng/mL

UniProt ID: P04179

Target Name: SOD2

Target Synonym: IPOB, IPO-B, MNSOD, Mn-SOD, MVCD6

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human SOD2. During the reaction, Human SOD2 in samples or Standard competes with a fixed amount of Human SOD2 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human SOD2. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Human SOD2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Human SOD2 in samples. No significant cross-reactivity or interference between Human SOD2 and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Human SOD2 concentrations in Serum, plasma and other biological fluids.

Research Areas: Cancer, Cell Biology, Cardiovascular, Metabolism, Neuroscience, Signal transduction

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Human SOD2 (Superoxide Dismutase 2, Mitochondrial) ELISA Kit DataSheet