Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit | G-EC-02273
- 3 to 5 Working Days
Human TNF-α (Tumor Necrosis Factor Alpha) ELISA Kit | G-EC-02273 | Gentaur Elisa Kits
Target Species: Human
Assay Time: 3.5h
Detection Type: Colormetric
Detection Range: 7.81~500pg/mL
UniProt ID: P01375
Target Name: TNF-α
Target Synonym: DIF, TNF-alpha, TNFA, TNFSF2
Tested Sample Types: Serum, plasma and other biological fluids
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TNF-α. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human TNF-α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TNF-α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human TNF-α. You can calculate the concentration of Human TNF-α in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Human TNF-α in samples. No significant cross-reactivity or interference between Human TNF-α and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Human TNF-α concentrations in Serum, plasma and other biological fluids.
Research Areas: Cancer, Cardiovascular, Metabolism, Immunology, Signal transduction
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.