LTB4 (Leukotriene B4) ELISA Kit | G-EC-00032

LTB4 (Leukotriene B4) ELISA Kit | G-EC-00032 | Gentaur Elisa Kits

Target Species: Universal

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 9.38pg/mL

Detection Range: 15.63~1000pg/mL

UniProt ID:

Target Name: LTB4

Target Synonym: Leukotriene B4

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with LTB4. During the reaction, LTB4 in samples or Standard competes with a fixed amount of LTB4 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to LTB4. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of LTB4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes LTB4 in samples. No significant cross-reactivity or interference between LTB4 and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of LTB4 concentrations in Serum, plasma and other biological fluids.

Research Areas: Cell Biology

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

LTB4 (Leukotriene B4) ELISA Kit DataSheet