Mouse αFP (Alpha-Fetoprotein) ELISA Kit | G-EC-04851
- 3 to 5 Working Days
Mouse αFP (Alpha-Fetoprotein) ELISA Kit | G-EC-04851 | Gentaur Elisa Kits
Target Species: Mouse
Assay Time: 3.5h
Detection Type: Colormetric
Detection Range: 0.16~10ng/mL
UniProt ID: P02772
Target Name: αFP
Target Synonym: AFP, FETA, HPAFP, Alpha-fetoglobulin, oralpha fetal protein
Tested Sample Types: Serum, plasma and other biological fluids
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse αFP. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse αFP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse αFP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Mouse αFP. You can calculate the concentration of Mouse αFP in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Mouse αFP in samples. No significant cross-reactivity or interference between Mouse αFP and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Mouse αFP concentrations in Serum, plasma and other biological fluids.
Research Areas: Cancer, Cardiovascular, Developmental biology
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.