Mouse GDH/GLDH (Glutamate dehydrogenase) ELISA Kit | G-EC-04416

Mouse GDH/GLDH (Glutamate dehydrogenase) ELISA Kit | G-EC-04416 | Gentaur Elisa Kits

Target Species: Mouse

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivity: 0.09mIU/mL

Detection Range: 0.16~10mIU/mL

UniProt ID: P26443

Target Name: GDH/GLDH

Target Synonym:

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse GDH/GLDH . Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse GDH/GLDH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse GDH/GLDH, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Mouse GDH/GLDH . You can calculate the concentration of Mouse GDH/GLDH in the samples by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Mouse GDH/GLDH in samples. No significant cross-reactivity or interference between Mouse GDH/GLDH and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Mouse GDH/GLDH concentrations in Serum, plasma and other biological fluids.

Research Areas: Cell Biology, Neuroscience, Metabolism

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Mouse GDH/GLDH (Glutamate dehydrogenase) ELISA Kit DataSheet