Mouse TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B) ELISA Kit | G-EC-04465

Mouse TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B) ELISA Kit | G-EC-04465 | Gentaur Elisa Kits

Target Species: Mouse

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivity: 4.69pg/mL

Detection Range: 7.81~500pg/mL

UniProt ID: P25119

Target Name: TNFRSF1B

Target Synonym: TNFR2, CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR80, p75, p75TNFR

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNFRSF1B. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse TNFRSF1B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNFRSF1B, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Mouse TNFRSF1B. You can calculate the concentration of Mouse TNFRSF1B in the samples by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Mouse TNFRSF1B in samples. No significant cross-reactivity or interference between Mouse TNFRSF1B and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Mouse TNFRSF1B concentrations in Serum, plasma and other biological fluids.

Research Areas: Cancer, Cell Biology, Cardiovascular, Immunology, Neuroscience, Signal transduction

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Mouse TNFRSF1B (Tumor Necrosis Factor Receptor Superfamily, Member 1B) ELISA Kit DataSheet