Pig Cortisol ELISA Kit | G-EC-00067

Pig Cortisol ELISA Kit | G-EC-00067 | Gentaur Elisa Kits

Target Species: Porcine

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 0.48ng/mL

Detection Range: 0.78~50ng/mL

UniProt ID:

Target Name: Cortisol

Target Synonym:

Tested Sample Types: Serum and plasma

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Porcine Cortisol. During the reaction, Porcine Cortisol in samples or Standard competes with a fixed amount of Porcine Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Porcine Cortisol. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Porcine Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Porcine Cortisol in samples. No significant cross-reactivity or interference between Porcine Cortisol and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Porcine Cortisol concentrations in Serum and plasma.

Research Areas: Signal transduction, Metabolism

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Pig Cortisol ELISA Kit DataSheet