Human Cortisol ELISA Kit | G-EC-00065
- 3 to 5 Working Days
Human Cortisol ELISA Kit | G-EC-00065 | Gentaur Elisa Kits
Target Species: Human
Assay Time: 2.5h
Detection Type: Colormetric
Detection Range: 6.25~400ng/mL
Target Name: Cortisol
Tested Sample Types: Serum, Plasma, Urine, Saliva
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human Cortisol. During the reaction, Human Cortisol in samples or Standard competes with a fixed amount of Human Cortisol on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human Cortisol. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Human Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Human Cortisol in samples. No significant cross-reactivity or interference between Human Cortisol and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Human Cortisol concentrations in Serum, Plasma, Urine, Saliva.
Research Areas: Signal transduction, Metabolism
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.