QuicKey Pro Human IL-6 (Interleukin 6) ELISA Kit | G-EC-06099
- 3 to 5 Working Days
QuicKey Pro Human IL-6 (Interleukin 6) ELISA Kit | G-EC-06099 | Gentaur Elisa Kits
Target Species: Human
Assay Time: 1.5h
Detection Type: Colormetric
Detection Range: 3.13-200pg/mL
UniProt ID: P05231
Target Name: IL-6
Target Synonym: Interleukin 6, IL6, BSF2, HGF, HSF, IFNB2, CDF
Tested Sample Types: Serum and plasma
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human IL-6. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human IL-6 are added to the micro ELISA plate wells. Human IL-6 in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human IL-6. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The OD value is proportional to the concentration of Human IL-6. The concentration of Human IL-6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Human IL-6 in samples. No significant cross-reactivity or interference between Human IL-6 and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Human IL-6 concentrations in Serum and plasma .
Research Areas: Cancer, Metabolism, Immunology, Neuroscience
Storage: An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.