QuicKey Pro Sheep T (Testosterone) ELISA Kit | G-EC-06129

QuicKey Pro Sheep T (Testosterone) ELISA Kit | G-EC-06129 | Gentaur Elisa Kits

Target Species: Sheep

Type: Competitive

Assay Time: 1.5h

Detection Type: Colormetric

Sensitivity: 0.25ng/mL

Detection Range: 0.63-40ng/mL

UniProt ID:

Target Name: T

Target Synonym: Testosterone，T

Tested Sample Types: Serum and plasma

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Sheep T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Sheep T are added to the micro ELISA plate wells. Sheep T in samples (or standards) competes with a fixed amount of Sheep T on the solid phase supporter for sites on the HRP linked detection antibody specific to Sheep T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Sheep T in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Sheep T in samples. No significant cross-reactivity or interference between Sheep T and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Sheep T concentrations in Serum and plasma .

Research Areas: Signal Transduction, Signal Transduction, Epigenetics and Nuclear Signaling, Developmental Biology

Storage: An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the storage table in the manual.

QuicKey Pro Sheep T (Testosterone) ELISA Kit DataSheet