Rat CRH (Corticotropin Releasing Hormone) ELISA Kit | G-EC-00170
- 3 to 5 Working Days
Rat CRH (Corticotropin Releasing Hormone) ELISA Kit | G-EC-00170 | Gentaur Elisa Kits
Target Species: Rat
Assay Time: 2.5h
Detection Type: Colormetric
Detection Range: 0.31~20ng/mL
UniProt ID: P01143
Target Name: CRH
Target Synonym: CRF, Corticoliberin
Tested Sample Types: Serum, plasma and other biological fluids
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat CRH. During the reaction, Rat CRH in samples or Standard competes with a fixed amount of Rat CRH on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat CRH. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Rat CRH in the samples is then determined by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Rat CRH in samples. No significant cross-reactivity or interference between Rat CRH and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Rat CRH concentrations in Serum, plasma and other biological fluids.
Research Areas: Cancer, Neuroscience, Signal transduction
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.