Rat β-CTx (Beta Crosslaps) ELISA Kit | G-EC-00190
- 3 to 5 Working Days
Rat β-CTx (Beta Crosslaps) ELISA Kit | G-EC-00190 | Gentaur Elisa Kits
Target Species: Rat
Assay Time: 2.5h
Detection Type: Colormetric
Detection Range: 125~8000pg/mL
Target Name: β-CTx
Target Synonym: b-CTx, bCTXI, bCTX-I, B-Cr, BCL, Type I Collagen C-Telopeptide-Related Fraction
Tested Sample Types: Serum, plasma and other biological fluids
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat β-CTx. During the reaction, Rat β-CTx in samples or Standard competes with a fixed amount of Rat β-CTx on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat β-CTx. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Rat β-CTx in the samples is then determined by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Rat β-CTx in samples. No significant cross-reactivity or interference between Rat β-CTx and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Rat β-CTx concentrations in Serum, plasma and other biological fluids.
Research Areas: Signal transduction, Metabolism
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.