Rat GzmA (Granzyme A) ELISA Kit | G-EC-05394

Rat GzmA (Granzyme A) ELISA Kit | G-EC-05394 | Gentaur Elisa Kits

Target Species: Rat

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivity: 0.94ng/mL

Detection Range: 1.56~100ng/mL

UniProt ID: G3V7E3

Target Name: GzmA

Target Synonym:

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat GzmA. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GzmA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GzmA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Rat GzmA. You can calculate the concentration of Rat GzmA in the samples by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Rat GzmA in samples. No significant cross-reactivity or interference between Rat GzmA and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Rat GzmA concentrations in Serum, plasma and other biological fluids.

Research Areas: Cancer, Cell Biology, Immunology

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Rat GzmA (Granzyme A) ELISA Kit DataSheet