Rat HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit | G-EC-05441
- 3 to 5 Working Days
Rat HIF-1α (Hypoxia Inducible Factor 1 Alpha) ELISA Kit | G-EC-05441 | Gentaur Elisa Kits
Target Species: Rat
Assay Time: 3.5h
Detection Type: Colormetric
Detection Range: 7.81~500pg/mL
UniProt ID: O35800
Target Name: HIF-1α
Target Synonym: HIF1A, HIF-1A, MOP1, PASD8, bHLHe78, basic helix-loop-helix transcription factor
Tested Sample Types: Serum, plasma and other biological fluids
Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat HIF-1α. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HIF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HIF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Rat HIF-1α. You can calculate the concentration of Rat HIF-1α in the samples by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Rat HIF-1α in samples. No significant cross-reactivity or interference between Rat HIF-1α and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Rat HIF-1α concentrations in Serum, plasma and other biological fluids.
Research Areas: Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.