Rat MT2 (Metallothionein 2) ELISA Kit | G-EC-05805

Rat MT2 (Metallothionein 2) ELISA Kit | G-EC-05805 | Gentaur Elisa Kits

Target Species: Rat

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivity: 0.19ng/mL

Detection Range: 0.31~20ng/mL

UniProt ID: P04355

Target Name: MT2

Target Synonym:

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MT2. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MT2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MT2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Rat MT2. You can calculate the concentration of Rat MT2 in the samples by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Rat MT2 in samples. No significant cross-reactivity or interference between Rat MT2 and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Rat MT2 concentrations in Serum, plasma and other biological fluids.

Research Areas: Cell Biology, Epigenetics and Nuclear Signal,

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Rat MT2 (Metallothionein 2) ELISA Kit DataSheet