Rat NOX4 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4) ELISA Kit | G-EC-05533

Rat NOX4 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4) ELISA Kit | G-EC-05533 | Gentaur Elisa Kits

Target Species: Rat

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivity: 0.19ng/mL

Detection Range: 0.31~20ng/mL

UniProt ID: Q924V1

Target Name: NOX4

Target Synonym: KOX, KOX-1, RENOX, NADPH Oxidase 4

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NOX4. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NOX4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NOX4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Rat NOX4. You can calculate the concentration of Rat NOX4 in the samples by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Rat NOX4 in samples. No significant cross-reactivity or interference between Rat NOX4 and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Rat NOX4 concentrations in Serum, plasma and other biological fluids.

Research Areas: Cancer, Cell Biology, Cardiovascular, Metabolism, Signal transduction

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Rat NOX4 (Nicotinamide Adenine Dinucleotide Phosphate Oxidase 4) ELISA Kit DataSheet