Rat OXB (Orexin B) ELISA Kit | G-EC-00179
- 3 to 5 Working Days
Rat OXB (Orexin B) ELISA Kit | G-EC-00179 | Gentaur Elisa Kits
Target Species: Rat
Assay Time: 2.5h
Detection Type: Colormetric
Detection Range: 0.16~10ng/mL
Target Name: OXB
Target Synonym: Hypocretin-2
Tested Sample Types: Serum, plasma and other biological fluids
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat OXB. During the reaction, Rat OXB in samples or Standard competes with a fixed amount of Rat OXB on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat OXB. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Rat OXB in the samples is then determined by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes Rat OXB in samples. No significant cross-reactivity or interference between Rat OXB and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of Rat OXB concentrations in Serum, plasma and other biological fluids.
Research Areas: Cancer, Metabolism, Neuroscience
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.