Rat PFK-A (6-phosphofructokinase Type A) ELISA Kit | G-EC-05814

Rat PFK-A (6-phosphofructokinase Type A) ELISA Kit | G-EC-05814 | Gentaur Elisa Kits

Target Species: Rat

Type: Sandwich

Assay Time: 3.5h

Detection Type: Colormetric

Sensitivity: 0.94ng/mL

Detection Range: 1.56~100ng/mL

UniProt ID:

Target Name: PFK-A

Target Synonym: PFK-M, ATP-PFK, ATP-dependent 6-phosphofructokinase, muscle type, Phosphofructo-1-kinase isozyme A, Phosphohexokinase

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PFK-A. Samples (or Standards) are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PFK-A and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PFK-A, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Rat PFK-A. You can calculate the concentration of Rat PFK-A in the samples by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Rat PFK-A in samples. No significant cross-reactivity or interference between Rat PFK-A and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Rat PFK-A concentrations in Serum, plasma and other biological fluids.

Research Areas: Metabolism, Signal Transduction, Cancer

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Rat PFK-A (6-phosphofructokinase Type A) ELISA Kit DataSheet