Rat PGF2α (Prostaglandin F2 Alpha) ELISA Kit | G-EC-00182

Rat PGF2α (Prostaglandin F2 Alpha) ELISA Kit | G-EC-00182 | Gentaur Elisa Kits

Target Species: Rat

Type: Competitive

Assay Time: 2.5h

Detection Type: Colormetric

Sensitivity: 4.69pg/mL

Detection Range: 7.81~500pg/mL

UniProt ID:

Target Name: PGF2α

Target Synonym:

Tested Sample Types: Serum, plasma and other biological fluids

Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat PGF2α. During the reaction, Rat PGF2α in samples or Standard competes with a fixed amount of Rat PGF2α on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat PGF2α. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of Rat PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.

Specificity: This kit recognizes Rat PGF2α in samples. No significant cross-reactivity or interference between Rat PGF2α and analogues was observed.

Application: This ELISA kit applies to the in vitro quantitative determination of Rat PGF2α concentrations in Serum, plasma and other biological fluids.

Research Areas: Cell Biology

Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.

Rat PGF2α (Prostaglandin F2 Alpha) ELISA Kit DataSheet