Recombinant Protein Expression

Recombinant Protein Expression Overview

Recombinant proteins are important tools used in the study of biological processes, molecular mechanisms, biotechnology research, and pharmaceutical development.

Protein expression systems enable researchers to produce specific proteins through engineered genetic constructs introduced into suitable host organisms. Commonly used expression systems include:

• Escherichia coli
• Yeast expression systems
• Baculovirus systems
• Insect cells
• Mammalian cell expression systems

The key steps of recombinant protein production include gene cloning, transfection, screening, and protein expression.

Recombinant Protein Expression Workflow

1. Vector

Gene vectors are essential tools for delivering target genes into cells. They allow the introduction and release of genetic material and, in some cases, enable integration into the host cell nucleus for expression of specific biological functions.

Based on their applications, gene vectors are mainly classified into:

• Cloning vectors: Used for DNA amplification and maintenance.
• Expression vectors: Designed for controlled production of recombinant proteins.

The pSmart™ series of expression vectors is compatible with systems such as E. coli and yeast, allowing researchers to select the most suitable vector according to experimental requirements.

2. Extraction and Amplification Kit

To facilitate efficient molecular cloning workflows, a comprehensive selection of nucleic acid recovery kits is available, including:

• Column-based purification systems
• Magnetic bead-based purification systems
• DNA recovery and molecular cloning solutions

These solutions simplify experimental procedures and improve the efficiency of recombinant DNA construction.

The DNA Assembly Cloning Kit enables directional cloning of inserted fragments into different vector sites using a seamless cloning method.

HS Universal qPCR Master Mix, ROX Plus

The HS Universal qPCR Master Mix, ROX Plus is a SYBR Green I-based quantitative PCR master mix designed for accurate and reproducible DNA quantification.

Figure 1. HS Universal qPCR Master Mix, ROX Plus for highly sensitive quantitative PCR applications.

This formulation incorporates antibody-mediated Hot-Start DNA polymerase and an optimized reaction buffer system to improve amplification specificity through controlled thermal activation.

Key Performance Advantages

• Enhanced amplification specificity: Reduces non-specific amplification through Hot-Start activation technology.
• High sensitivity: Provides improved detection performance for low-copy DNA targets.
• Wide quantification range: Supports accurate measurements across different template concentrations.
• Excellent reproducibility: Maintains consistent results between experimental replicates.
• ROX reference dye included: Compatible with qPCR instruments requiring fluorescence signal normalization.

DNA Assembly Cloning Kit

The DNA Assembly Cloning Kit is a rapid, simple, and efficient seamless cloning solution designed for directional insertion of DNA fragments into vectors.

The kit allows researchers to linearize vectors using different methods and introduce vector-specific sequences into the 5′ ends of forward and reverse primers.

Cloning Principle

1. The target vector is linearized using an appropriate method.
2. Vector end sequences are added to PCR primers during amplification.
3. PCR products containing homologous sequences are mixed with the linearized vector.
4. Recombinase-mediated assembly completes directional cloning.
5. The reaction can be transformed after incubation at approximately 50°C for 15 minutes.

3. Culture Medium

For prokaryotic expression systems, exogenous gene expression typically requires induction. To simplify recombinant protein production workflows, ready-to-use culture media solutions are available.

These culture media options reduce preparation steps and can be directly used during the induction stage without additional inducer preparation.

For CHO mammalian expression systems, serum-free media and feed solutions are provided to support efficient eukaryotic protein production.

Figure 2. Culture media suitable for recombinant protein expression in E. coli.

Ordering Information

Explore Recombinant Protein Expression Products

Discover vectors, cloning kits, qPCR reagents, and culture media solutions designed for efficient recombinant protein production workflows.