Recombinant Protein

 

 

E. coli expressed proteins form Gentaur

 

CAS lab uses several up to 4 purification steps to purify our recombinants.

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Escherichia coli produces recombinant proteins.

This cell factory used for the synthesis of recombinant proteins in E. coli and plasmids.

Recombinant proteins whare first made by Peprotec Ltd. that took the gene of interest, cloned it in whatever expression vector then, the proteins where ready for HPLC purification and characterization by cell culture.

Peprotech did not make all the recombinants themselves. They exchanged most of the cytokines in their catalog with other researchers. Int his way having some proteins they made the biggest catalog available in 1997.

In practice, however, lots of factors can go wrong. Poor growth of the host, inclusion body (IB) formation, protein inactivity, and even not obtaining any protein at all. Glycosilation is better in Pychia or CHO recombinants than in proteins from E. Coli expression.

CAS Lab is a leader in dealing with difficult-to-express proteins.

Inclusion bodies (IBs), protein aggregates inside of the cytoplasm of the cell publication by C Slouka.

  • Recombinant Human BFGF
    Gentaur

    Recombinant Human BFGF

    Recombinant Human BFGF Description: Human basic Fibroblast Growth Factor (bFGF) is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. bFGF has been shown to...
    $325.00
  • Recombinant Human EGF
    Gentaur

    Recombinant Human EGF

    Recombinant Human EGF Description: Recombinant human epidermal growth factor is 6.2kDa globular protein comprosed of 53 amino acids. EGF is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells...
    $253.00
  • Filgrastim (rHuG-CSF)
    Gentaur

    Filgrastim (rHuG-CSF)

    Filgrastim (rHuG-CSF) Description: Filgrastim is a recombinant human granulocyte colony stimulating factor (rHuG-CSF), produced by recombinant DNA technology using E.coli. It is a 175 amino acid protein, non-glycosylated, and has a molecular weight of...
    $328.00 - $376.00
  • Molgramostim (rHuGM-CSF)
    Gentaur

    Molgramostim (rHuGM-CSF)

    Molgramostim (rHuGM-CSF) Description: Granulocyte/Macrophage Colony-Stimulating Factor (GM-CSF) was initially characterized as a factor that can support the in vitro colony formation of granulocyte macrophage progenitors. It is also a growth factor for...
    $315.00 - $411.00
  • Recombinant Human IFN-γ
    Gentaur

    Recombinant Human IFN-γ

    Recombinant Human IFN-γ Description: Interferon gamma (IFN-γ) is an acid-labile interferon produced by CD4 and CD8 T lymphocytes as well as activated NK cells. IFN-γ receptors are present in most immune cells, which respond to IFN-γ signaling by...
    $325.00 - $463.00
  • Recombinant Human IFN α-2b
    Gentaur

    Recombinant Human IFN α-2b

    Recombinant Human IFN α-2b Description: Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon...
    $325.00 - $463.00
  • Recombinant Human IFN α-2a
    Gentaur

    Recombinant Human IFN α-2a

    Recombinant Human IFN α-2a Description: Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon...
    $325.00 - $463.00
  • Recombinant Human IL-2
    Gentaur

    Recombinant Human IL-2

    Recombinant Human IL-2 Description: The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided...
    $325.00 - $463.00