SARS-CoV-2 Spike Protein Total Antibody ELISA Kit | G-EC-02192
- 3 to 5 Working Days
SARS-CoV-2 Spike Protein Total Antibody ELISA Kit | G-EC-02192 | Gentaur Elisa Kits
Target Species: Universal
Assay Time: 3.5h
Detection Type: Colormetric
Target Name: Spike Protein Total Antibodies
Target Synonym: COVID 19 Spike Protein Total Antibody, Spike Protein Total Antibody, Spike Protein Antibody, SP Total Antibody, SP Antibody，SARS-CoV-2 Spike (RBD) Tab、2019-nCoV Spike (RBD) Tab、COVID-19 Spike (RBD) Tab
Tested Sample Types: Serum and plasma
Test Principle: This ELISA kit uses the Sandwich-Ag ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with recombinant SARS-CoV-2 Spike Protein. After adding samples (or controls) to wells, the total antibodies against SARS-CoV-2 Spike Protein will combine with the pre-coated SARS-CoV-2 Spike Protein. After washing completely, add Horseradish Peroxidase (HRP) conjugated SARS-CoV-2 Spike Protein to develop the antigen-antibody-HRP conjugated antigen complex. Free components are washed away. The substrate solution is added to each well. Only those wells that contain SARS-CoV-2 Spike Protein, antibodies against SARS-CoV-2 Spike Protein and HRP conjugated SARS-CoV-2 Spike Protein will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. Compared with the CUT OFF value to judge whether SARS-CoV-2 Spike Protein total antibody exists in the tested samples or not.
Specificity: This kit recognizes Spike Protein Total Antibodies in samples. No significant cross-reactivity or interference between Spike Protein Total Antibodies and analogues was observed.
Application: This ELISA kit applies to the in vitro qualitative determination of total antibodies against SARS-CoV-2 Spike Protein in serum or plasma.
Research Areas: SARS-CoV-2, COVID 19
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.