VB6 (Vitamin B6) ELISA Kit | G-EC-00001
- 3 to 5 Working Days
VB6 (Vitamin B6) ELISA Kit | G-EC-00001 | Brand Name Kits
Target Species: Universal
Assay Time: 2.5h
Detection Type: Colormetric
Detection Range: 1.56~100ng/mL
Target Name: VB6
Tested Sample Types: Serum, plasma and other biological fluids
Test Principle: This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with VB6. During the reaction, VB6 in samples or Standard competes with a fixed amount of VB6 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to VB6. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase (HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The concentration of VB6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Specificity: This kit recognizes VB6 in samples. No significant cross-reactivity or interference between VB6 and analogues was observed.
Application: This ELISA kit applies to the in vitro quantitative determination of VB6 concentrations in Serum, plasma and other biological fluids.
Research Areas: Signal transduction, Metabolism
Storage: An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the items separately according to the storage table in the manual once the kit is received.