ADO Polyclonal Antibody | G-AB-02691

ADO Polyclonal Antibody | G-AB-02691 | Gentaur Antibodies

Overview: Human thiol dioxygenases include cysteine dioxygenase (CDO, MIM 603943) and cysteamine (2-aminoethanethiol) dioxygenase (ADO, EC 1.13.11.19) . CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. Mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. The demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.

Category Type: Polyclonal Antibody

Research Areas: Metabolism, Signal Transduction

Synonyms: 2 aminoethanethiol dioxygenase, 2-aminoethanethiol dioxygenase, ADO 2 aminoethanethiol (cysteamine) dioxygenase, ADO, AEDO, C10orf22, Chromosome 10 open reasing frame 22, cysteamine (2-aminoethanethiol) dioxygenase (ADO) , Cysteamine dioxygenase, DKFZp564C046, FLJ14547

Reactivity: Human, Mouse

Host: Rabbit

Isotype: IgG

Gene ID:

Accession #: NP_116193

Clonality: Polyclonal

Immunogen: Synthetic peptide of human ADO

Clone #:

Conjugation: Unconjugated

Swissprot: Q96SZ5

Santa Cruz: sc-162492/sc-162493

Calculated MW: 30 kDa

Observed MW:

Concentration: 0.3 mg/mL

Buffer: PBS with 0.05% sodium azide and 50% glycerol, PH7.4

Purification method: Affinity purification

Application: WB, IHC, ELISA

Dilution: WB 1:1000-1:5000, IHC 1:25-1:100

Storage: Store at -20°C. Avoid freeze / thaw cycles.

ADO Polyclonal Antibody DataSheet