beta actin Polyclonal Antibody | G-AB-00295

beta actin Polyclonal Antibody | G-AB-00295 | Gentaur Antibodies

Overview: Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1) , alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle) , beta (ACTB) , gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle) . Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1 (found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

Category Type: Control Antibody

Research Areas: Isotype, Tags & Cell Markers, Signal Transduction

Synonyms: ACTB, Actin beta, Actx, Actin cytoplasmic 1, b actin, A26C1A

Reactivity: Human, Mouse, Rat

Host: Rabbit

Isotype: IgG

Gene ID: 57935

Accession #:

Clonality: Polyclonal

Immunogen: Recombinant Zebrafish Actin, cytoplasmic 2 protein

Clone #:

Conjugation: Unconjugated

Swissprot: Q7ZVF9

Santa Cruz:

Calculated MW: 41 kDa

Observed MW: 41 kDa

Concentration: 0.85 mg/mL

Buffer: PBS with 0.05% Proclin300, 50% glycerol, pH7.3

Purification method: Antigen Affinity Purification

Application: WB, IHC

Dilution: WB 1:1000-1:2000 IHC 1:100-1:300

Storage: Store at -20°C. Avoid freeze / thaw cycles.

beta actin Polyclonal Antibody DataSheet

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