beta actin Polyclonal Antibody | G-AB-05810
- 3 to 5 Working Days
- Human, Mouse, Rat
beta actin Polyclonal Antibody | G-AB-05810 | Gentaur Antibodies
Overview: Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1) , alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle) , beta (ACTB) , gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle) . Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1 (found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.
Category Type: Control Antibody
Research Areas: Cancer, Signal Transduction, Tags & Cell Markers
Synonyms: A26C1A, A26C1B, ACTB, ACTB, Actin beta, Actin cytoplasmic 1, Actin, cytoplasmic 1, N-terminally processed, Actx, b actin, Beta cytoskeletal actin, Beta-actin, BRWS1, E430023M04Rik, MGC128179, PS1TP5 binding protein 1, PS1TP5BP1
Reactivity: Human, Mouse, Rat
Immunogen: Synthesized peptide derived from the N-terminal region of human Actin β.
Calculated MW: 42 kDa
Observed MW: 42 kDa
Concentration: 1 mg/mL
Buffer: PBS with 0.02% sodium azide, 0.5% BSA and 50% glycerol, pH7.4
Purification method: Affinity purification
Application: WB, IHC-p, ELISA
Dilution: WB 1:1000-1:4000, IHC 1:100-1:300, ELISA 1:20000
Storage: Store at -20°C. Avoid freeze / thaw cycles.