Flag-Tag Polyclonal Antibody | G-AB-05794

Flag-Tag Polyclonal Antibody | G-AB-05794 | Gentaur Antibodies

Overview: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK (FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal FLAG vectors provide an Ek cleavage site for removal of the fusion tag. The FLAG peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the FLAG peptide is more likely to be accessible to antibodies.

Category Type: Tag Antibody

Research Areas: Tags & Cell Markers

Synonyms: DDDDK epitope tag, DDDDK epitope tag, DYKDDDDK epitope tag

Reactivity:

Host: Rabbit

Isotype: IgG

Gene ID:

Accession #:

Clonality: Polyclonal

Immunogen: Synthetic Peptide

Clone #:

Conjugation: Unconjugated

Swissprot:

Santa Cruz:

Calculated MW:

Observed MW:

Concentration: 1 mg/mL

Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

Purification method: Affinity purification

Application: WB

Dilution: WB 1:5000-20000

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Flag-Tag Polyclonal Antibody DataSheet